Journal: Laboratory Animal Research

Article Title: Comparison of intrinsic exercise capacity and response to acute exercise in ICR (Institute of Cancer Research) mice derived from three different lineages

doi: 10.1186/s42826-021-00094-0

Figure Lengend Snippet: Comparative analysis of the cardiac mitochondrial oxidative phosphorylation (OXPHOS) complex, mitochondrial function, and uncoupling protein (UCP) 3 expression levels among the ICR lineages. A Representative western blots showing the protein levels of the OXPHOS complex. B The densitometric analysis of blots normalized for COX-IV in the cardiac mitochondrial fraction of different ICR lineages. C Oxygen consumption rate (OCR) in the permeabilized cardiac muscles of different ICR lineages was recorded using the Oroboros O2K instrument. D The respiratory control ratio (RCR) was calculated as the ratio of oxygen consumption at state 3 (complex 1 and complex 1 + 2) to that at state 2. E Representative western blots showing the protein levels of UCP3 in the soleus, extensor digitorum longus (EDL), and cardiac muscle. F The densitometric analysis of blots normalized for the density of Ponceau staining in different muscles. The values are presented as mean ± SEM; each group consisted of 5–6 mice. * P < .05, ** P < .01, and *** P < .001

Article Snippet: C Oxygen consumption rate (OCR) in the permeabilized cardiac muscles of different ICR lineages was recorded using the Oroboros O2K instrument.

Techniques: Phospho-proteomics, Expressing, Western Blot, Muscles, Control, Staining

Journal: Laboratory Animal Research

Article Title: Comparison of intrinsic exercise capacity and response to acute exercise in ICR (Institute of Cancer Research) mice derived from three different lineages

doi: 10.1186/s42826-021-00094-0

Figure Lengend Snippet: Comparative analysis of the metabolic phenotypes of different ICR lineages during a 24-h period at resting metabolic rate (RMR). A Schematics of the experimental design and protocol. B Changes in oxygen uptake and total oxygen uptake during different phases (total, inactive, and active). C Changes in CO 2 production and total CO 2 production during different phases. D Changes in the respiratory exchange ratio (RER) and average RER during different phases. The shaded part of the line graph represents the dark period of the day cycle. The values are presented as mean ± SEM; each group consisted of four mice

Article Snippet: C Oxygen consumption rate (OCR) in the permeabilized cardiac muscles of different ICR lineages was recorded using the Oroboros O2K instrument.

Techniques:

Journal: The Journal of Biological Chemistry

Article Title: A CRISPR-edited isoform of the AMPK kinase LKB1 improves the response to cisplatin in A549 lung cancer cells

doi: 10.1016/j.jbc.2025.108308

Figure Lengend Snippet: Super LKB1 enhances the oxidative metabolism in A549-edited cells. A , Western blotting of HK2, LDHA, and GAPDH in cWT, c2SL+, and c3SL + cells; ( B ) normalized levels of proteins by GAPDH (a.u); ( C ) Western blotting of OXPHOS and GAPDH in cWT, c2SL+, and c3SL + cells. D , normalized levels of proteins by GAPDH (a.u); ( E ) oxygen consumption rate (OCR) by OROBOROS in cWT, c2SL+, and c3SL + cells; ( F ) comparison levels of O 2 in the cell lines in the basal condition and after treatments with oligomycin and FCCP. The data are presented as mean ± SD. Statistical analysis was performed by ANOVA followed by Dunnet’s test ∗ p < 0.05, ∗∗ p < 0.01. These data are representative of two independent experiments.

Article Snippet: D , normalized levels of proteins by GAPDH (a.u); ( E ) oxygen consumption rate (OCR) by OROBOROS in cWT, c2SL+, and c3SL + cells; ( F ) comparison levels of O 2 in the cell lines in the basal condition and after treatments with oligomycin and FCCP.

Techniques: Western Blot, Comparison

Journal: Biomolecules

Article Title: Catalpol Ameliorates Insulin Sensitivity and Mitochondrial Respiration in Skeletal Muscle of Type-2 Diabetic Mice Through Insulin Signaling Pathway and AMPK/SIRT1/PGC-1α/PPAR-γ Activation

doi: 10.3390/biom10101360

Figure Lengend Snippet: Effect of catalpol on state-3/stae-4 respiration, oxygen consumption rate (OCR) and respiratory exchange rate (RCR) ( A ) Representative trace of oxygen consumption shown by relative fluorescent units (RFU) over time between 0 and 10 min. ( B ) Oxygen onsumptionrate of isolated mitochondria from skeletal muscle. The slopes of the oxygen consumption curves were measured between 0 and 10 min; ( C ) Respiratory Control Ratio (RCR) was calculated as the ratio of State 3 versus State 4 respiration slope. NORM: Normal control; DIA: Diabetic control; CAT_200: Catalpol (200 mg/kg; p.o.); MET_200: Metformin (200 mg/kg, p.o.). Data represents mean ± SEM ( n = 3 per group as pooled sample). # p < 0.05 and ## p < 0.01 vs. NORM; * p < 0.05 and ** p < 0.01 vs. DIA.

Article Snippet: Oxygen consumption rate was measured using MitoCheck ® Mitochondrial oxygen consumption rate (OCR) assay kit (Cayman Chemical, Ann Arbor, MI, USA) according to the manufacturer’s protocol.

Techniques: Isolation, Control

Journal: Cell Metabolism

Article Title: Endothelial Lactate Controls Muscle Regeneration from Ischemia by Inducing M2-like Macrophage Polarization

doi: 10.1016/j.cmet.2020.05.004

Figure Lengend Snippet: Endothelial Lactate Controls Macrophage Polarization and Function upon Muscle Ischemia (A) Scheme illustrating experimental set-up. (B) Gene profiling of unstimulated BMDMs (vehicle) or BMDMs stimulated with mECs wt -CM, mECs Δpfkfb3 -CM. (C and D) Gene expression analysis of arg1 (C) and mrc1 (D) in BMDMs stimulated with fractionated mECs wt -CM and mECs Δpfkfb3 -CM, supplemented with lactate (5 mM) where indicated. (E) Lactate concentration in mECs wt -CM and mECs Δpfkfb3 -CM. (F) Representative images of immunostainings of F4/80 (green), CD206 (red), and hoechst (blue) in BMDMs stimulated with mECs wt -CM, mECs Δpfkfb3 -CM with or without lactate supplementation and quantification of CD206 MFI (n = 3). (G) OCR upon injection of oligomycin (oligo), FCCP, and rotenone plus antimycin A after mECs wt -CM, mECs Δpfkfb3 -CM, mECs wt+lac -CM, and mECs Δpfkfb3+lac -CM stimulation (n = 4–5). (H) RNaseq data showing OXPHOS gene expression in muscle macrophages 3 days after HLI (n = 3). (I and J) Representative images (I) and quantification (J) of proliferating MPCs upon incubation with macrophage-derived CM, measured as percentage of EdU + nuclei (red, EdU + ; blue, hoechst). (K and L) MPC fusion analysis: representative images of immunofluorescent DESMIN staining (K) (red, DESMIN; blue, hoechst) and quantification of the number of nuclei per DESMIN + myotube (L). (M) Vegf gene expression in CD45 + cells sorted from pfkfb3 WT and pfkfb3 ΔEC muscle at 3 d. (N) VEGF secretion by BMDMs after stimulation with mECs wt -CM and mECs Δpfkfb3 -CM with or without lactate supplementation. Scale bar, 50 μm. Student’s t test (two-tailed, unpaired) in (E) and (M). One-way ANOVA with Tukey’s multiple comparisons test in (B). Two-way ANOVA with Tukey’s multiple comparisons test in (C), (D), (G), (J), (L), and (N) ( ∗ p < 0.05) as well as in (F) ( ∗ p < 0.05 versus mECs wt -CM; # p < 0.05 versus ctrl). Each dot represents a single mouse (M) or the average of an independent experiment ([B], [C], [D], [E], [J], [L], and [N]). Bar graphs represent mean ± SEM. See also and .

Article Snippet: Because lactate, after conversion to pyruvate, can enter the tricarboxylic acid (TCA) cycle via pyruvate dehydrogenase, we also evaluated whether angiocrine lactate would promote oxygen consumption rate (OCR) in BMDMs.

Techniques: Gene Expression, Concentration Assay, Injection, Incubation, Derivative Assay, Staining, Two Tailed Test